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goat anti vcam1  (R&D Systems)


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    R&D Systems goat anti vcam1
    Goat Anti Vcam1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+vcam1/Mouse+VCAM-1%2FCD106+Antibody/pmc12354736-325-60-63
    Average 93 stars, based on 83 article reviews
    goat anti vcam1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Spatial transcriptomics defines injury specific microenvironments and cellular interactions in kidney regeneration and disease
    Article Snippet: .. Immunofluorescence and RNAscope experiments were performed as previously described . using the following antibodies and probes: mouse IgG2a anti-alpha Smooth Muscle Actin (aSMA, 1:2000, Sigma-Aldrich, A5228), goat anti-Vcam1 (1:200, R&D Systems, AF643), rabbit anti-Vcam1 (1:200, Abcam, ab134047), donkey anti-rabbit AlexaFluor 594 (1:500, Molecular probes, A21207), donkey anti-goat AlexaFlour 594 (1:500, Life Technologies, A11058), chick anti-rat AlexaFluor 647 (1:500, Molecular Probes, A21472). .. RNAscope Mm-Acta2 (ACD, 319531), RNAscope Mm-Cd4-C3 (ACD, 406841-C3), RNAscope Mm-Cd8a-C2 (ACD, 401681-C2), RNAscope Probe - Mm-Clcf1-C1 (ACD, 457971), RNAscope Mm-Clec9a-O1 (ACD, 537731), RNAscope Probe - Mm-Cntfr-C3 (ACD, 457981-C3), RNAscope Probe - Mm-Crlf1-C2 (ACD, 446891-C2), RNAscope Probe - Mm-Npr3-C3 (ACD, 502991-C3).

    Article Title: Spatial transcriptomics defines injury specific microenvironments and cellular interactions in kidney regeneration and disease.
    Article Snippet: Then, primary rabbit anti-Vcam1 antibody (Abcam, ab134047) was introduced at a 1:100 dilution in the blocking buffer and the sample was incubated at room temperature for 1 hr, washed three times in 1X PBS and a secondary anti-rabbit 647 antibody (Invitrogen, A32733) applied at 1:500 dilution for 1 hr at room temperature. .. Immunofluorescence and RNAscope experiments were performed as previously described19. using the following antibodies and probes: mouse IgG2a anti-alpha Smooth Muscle Actin (aSMA, 1:2000, SigmaAldrich, A5228), goat anti-Vcam1 (1:200, R&D Systems, AF643), rabbit anti-Vcam1 (1:200, Abcam, ab134047), donkey anti-rabbit AlexaFluor 594 (1:500, Molecular probes, A21207), donkey anti-goat AlexaFlour 594 (1:500, Life Technologies, A11058), chick anti-rat AlexaFluor 647 (1:500, Molecular Probes, A21472). .. RNAscope Mm-Acta2 (ACD, 319531), RNAscope Mm-Cd4-C3 (ACD, 406841-C3), RNAscope MmCd8a-C2 (ACD, 401681-C2), RNAscope Probe - Mm-Clcf1-C1 (ACD, 457971), RNAscope Mm-Clec9a-O1 (ACD, 537731), RNAscope Probe - Mm-Cntfr-C3 (ACD, 457981-C3), RNAscope Probe - Mm-Crlf1-C2 (ACD, 446891-C2), RNAscope Probe -Mm-Npr3-C3 (ACD, 502991-C3).

    Article Title: Spatial transcriptomics defines injury-specific microenvironments in the adult mouse kidney and novel cellular interactions in regeneration and disease
    Article Snippet: .. Immunofluorescence and RNAscope Immunofluorescence and RNAscope experiments were performed as previously described (19). using the following antibodies and probes: mouse IgG2a anti-alpha Smooth Muscle Actin (aSMA, 1:2000, Sigma-Aldrich, A5228), goat anti-Vcam1 (1:200, R&D Systems, AF643), rabbit antiVcam1 (1:200, Abcam, ab134047), donkey anti-rabbit AlexaFluor 594 (1:500, Molecular probes, A21207), donkey anti-goat AlexaFlour 594 (1:500, Life Technologies, A11058), chick anti-rat AlexaFluor 647 (1:500, Molecular Probes, A21472). .. RNAscope Mm-Acta2 (ACD, 319531), RNAscope Mm-Cd4-C3 (ACD, 406841-C3), RNAscope Mm-Cd8a-C2 (ACD, 401681-C2), RNAscope Probe - Mm-Clcf1-C1 (ACD, 457971), RNAscope Mm-Clec9a-O1 (ACD, 537731), RNAscope Probe - Mm-Cntfr-C3 (ACD, 457981-C3), RNAscope Probe - Mm-Crlf1-C2 (ACD, 446891-C2), RNAscope Probe - Mm-Npr3-C3 (ACD, 502991-C3).

    Article Title: Spatial transcriptomics defines injury-specific microenvironments in the adult mouse kidney and novel cellular interactions in regeneration and disease
    Article Snippet: .. Immunofluorescence and RNAscope experiments were performed as previously described ( ). using the following antibodies and probes: mouse IgG2a anti-alpha Smooth Muscle Actin (aSMA, 1:2000, Sigma-Aldrich, A5228), goat anti-Vcam1 (1:200, R&D Systems, AF643), rabbit anti-Vcam1 (1:200, Abcam, ab134047), donkey anti-rabbit AlexaFluor 594 (1:500, Molecular probes, A21207), donkey anti-goat AlexaFlour 594 (1:500, Life Technologies, A11058), chick anti-rat AlexaFluor 647 (1:500, Molecular Probes, A21472). .. RNAscope Mm-Acta2 (ACD, 319531), RNAscope Mm-Cd4-C3 (ACD, 406841-C3), RNAscope Mm-Cd8a-C2 (ACD, 401681-C2), RNAscope Probe - Mm-Clcf1-C1 (ACD, 457971), RNAscope Mm-Clec9a-O1 (ACD, 537731), RNAscope Probe - Mm-Cntfr-C3 (ACD, 457981-C3), RNAscope Probe - Mm-Crlf1-C2 (ACD, 446891-C2), RNAscope Probe - Mm-Npr3-C3 (ACD, 502991-C3).

    RNAscope:

    Article Title: Spatial transcriptomics defines injury specific microenvironments and cellular interactions in kidney regeneration and disease
    Article Snippet: .. Immunofluorescence and RNAscope experiments were performed as previously described . using the following antibodies and probes: mouse IgG2a anti-alpha Smooth Muscle Actin (aSMA, 1:2000, Sigma-Aldrich, A5228), goat anti-Vcam1 (1:200, R&D Systems, AF643), rabbit anti-Vcam1 (1:200, Abcam, ab134047), donkey anti-rabbit AlexaFluor 594 (1:500, Molecular probes, A21207), donkey anti-goat AlexaFlour 594 (1:500, Life Technologies, A11058), chick anti-rat AlexaFluor 647 (1:500, Molecular Probes, A21472). .. RNAscope Mm-Acta2 (ACD, 319531), RNAscope Mm-Cd4-C3 (ACD, 406841-C3), RNAscope Mm-Cd8a-C2 (ACD, 401681-C2), RNAscope Probe - Mm-Clcf1-C1 (ACD, 457971), RNAscope Mm-Clec9a-O1 (ACD, 537731), RNAscope Probe - Mm-Cntfr-C3 (ACD, 457981-C3), RNAscope Probe - Mm-Crlf1-C2 (ACD, 446891-C2), RNAscope Probe - Mm-Npr3-C3 (ACD, 502991-C3).

    Article Title: Spatial transcriptomics defines injury specific microenvironments and cellular interactions in kidney regeneration and disease.
    Article Snippet: Then, primary rabbit anti-Vcam1 antibody (Abcam, ab134047) was introduced at a 1:100 dilution in the blocking buffer and the sample was incubated at room temperature for 1 hr, washed three times in 1X PBS and a secondary anti-rabbit 647 antibody (Invitrogen, A32733) applied at 1:500 dilution for 1 hr at room temperature. .. Immunofluorescence and RNAscope experiments were performed as previously described19. using the following antibodies and probes: mouse IgG2a anti-alpha Smooth Muscle Actin (aSMA, 1:2000, SigmaAldrich, A5228), goat anti-Vcam1 (1:200, R&D Systems, AF643), rabbit anti-Vcam1 (1:200, Abcam, ab134047), donkey anti-rabbit AlexaFluor 594 (1:500, Molecular probes, A21207), donkey anti-goat AlexaFlour 594 (1:500, Life Technologies, A11058), chick anti-rat AlexaFluor 647 (1:500, Molecular Probes, A21472). .. RNAscope Mm-Acta2 (ACD, 319531), RNAscope Mm-Cd4-C3 (ACD, 406841-C3), RNAscope MmCd8a-C2 (ACD, 401681-C2), RNAscope Probe - Mm-Clcf1-C1 (ACD, 457971), RNAscope Mm-Clec9a-O1 (ACD, 537731), RNAscope Probe - Mm-Cntfr-C3 (ACD, 457981-C3), RNAscope Probe - Mm-Crlf1-C2 (ACD, 446891-C2), RNAscope Probe -Mm-Npr3-C3 (ACD, 502991-C3).

    Article Title: Spatial transcriptomics defines injury-specific microenvironments in the adult mouse kidney and novel cellular interactions in regeneration and disease
    Article Snippet: .. Immunofluorescence and RNAscope Immunofluorescence and RNAscope experiments were performed as previously described (19). using the following antibodies and probes: mouse IgG2a anti-alpha Smooth Muscle Actin (aSMA, 1:2000, Sigma-Aldrich, A5228), goat anti-Vcam1 (1:200, R&D Systems, AF643), rabbit antiVcam1 (1:200, Abcam, ab134047), donkey anti-rabbit AlexaFluor 594 (1:500, Molecular probes, A21207), donkey anti-goat AlexaFlour 594 (1:500, Life Technologies, A11058), chick anti-rat AlexaFluor 647 (1:500, Molecular Probes, A21472). .. RNAscope Mm-Acta2 (ACD, 319531), RNAscope Mm-Cd4-C3 (ACD, 406841-C3), RNAscope Mm-Cd8a-C2 (ACD, 401681-C2), RNAscope Probe - Mm-Clcf1-C1 (ACD, 457971), RNAscope Mm-Clec9a-O1 (ACD, 537731), RNAscope Probe - Mm-Cntfr-C3 (ACD, 457981-C3), RNAscope Probe - Mm-Crlf1-C2 (ACD, 446891-C2), RNAscope Probe - Mm-Npr3-C3 (ACD, 502991-C3).

    Article Title: Spatial transcriptomics defines injury-specific microenvironments in the adult mouse kidney and novel cellular interactions in regeneration and disease
    Article Snippet: .. Immunofluorescence and RNAscope experiments were performed as previously described ( ). using the following antibodies and probes: mouse IgG2a anti-alpha Smooth Muscle Actin (aSMA, 1:2000, Sigma-Aldrich, A5228), goat anti-Vcam1 (1:200, R&D Systems, AF643), rabbit anti-Vcam1 (1:200, Abcam, ab134047), donkey anti-rabbit AlexaFluor 594 (1:500, Molecular probes, A21207), donkey anti-goat AlexaFlour 594 (1:500, Life Technologies, A11058), chick anti-rat AlexaFluor 647 (1:500, Molecular Probes, A21472). .. RNAscope Mm-Acta2 (ACD, 319531), RNAscope Mm-Cd4-C3 (ACD, 406841-C3), RNAscope Mm-Cd8a-C2 (ACD, 401681-C2), RNAscope Probe - Mm-Clcf1-C1 (ACD, 457971), RNAscope Mm-Clec9a-O1 (ACD, 537731), RNAscope Probe - Mm-Cntfr-C3 (ACD, 457981-C3), RNAscope Probe - Mm-Crlf1-C2 (ACD, 446891-C2), RNAscope Probe - Mm-Npr3-C3 (ACD, 502991-C3).

    Blocking Assay:

    Article Title: Supporting Information for Multiomics profiling of mouse polycystic kidney disease progression at a single-cell resolution
    Article Snippet: The slides were placed in antigen retrieval solution (Vector H-330) and incubated in a pressure cooker. .. The sections were blocked in blocking buffer [1% BSA (Roche; 03116956001), 0.1% Triton X-100 (Sigma; T8787) in PBS] for 1 h, and the following primary antibodies or glycoproteins were added (1:200); LTL (Vector Labs; B-1325 or FL1321), DBA (Vector Labs; B-1035 or FL-1031), sheep Anti-UMOD (Bio-Rad; 8595-0054), rabbit Anti-Calbindin1 (Abcam; ab108404), goat Anti-AQP2 (Novus Biologicals; NBP1-70378), rabbit Anti-TMSB4X (Immunodiagnostik; A9520.2), goat Anti-VCAM1 (R&D Systems; AF643-SP), rabbit Anti-VCAM1 (Abcam; ab134047), rabbit Anti-SPP1(Abcam; ab218237), rabbit Anti-GPRC5A (Novus Biologicals; NBP1-89743) and incubated overnight at 4°C. .. The next day, slides were wash with PBS, and secondary antibodies in blocking buffer were added (1:200); donkey Anti-Goat (Invitrogen; A11057 or A32814), donkey Anti-Mouse (Invitrogen; A10037), donkey Anti-Rabbit (Invitrogen; A10042 or Jackson ImmunoResearch; 711-545-152), donkey Anti-Sheep antibody (Jackson ImmunoResearch; 713-165-147), conjugated streptavidin (Invitrogen; S21374) and incubated at room temperature for 1 h. Sections were stained with DAPI (4 ,́6 ́- diamidino-2phenylindole) and mounted in Prolong Gold (Life Technologies).

    Article Title: Multi-omics profiling of mouse polycystic kidney disease progression at a single cell resolution
    Article Snippet: The slides were placed in antigen retrieval solution (Vector H-330) and incubated in a pressure cooker. .. The sections were blocked in blocking buffer [1% BSA (Roche; 03116956001), 0.1% Triton X-100 (Sigma; T8787) in PBS] for 1 h, and the following primary antibodies or glycoproteins were added (1:200); LTL (Vector Labs; B-1325 or FL-1321), DBA (Vector Labs; B-1035 or FL-1031), sheep Anti-UMOD (Bio-Rad; 8595-0054), rabbit Anti-Calbindin1 (Abcam; ab108404), goat Anti-AQP2 (Novus Biologicals; NBP1-70378), rabbit Anti-TMSB4X (Immunodiagnostik, A9520.2), goat Anti-VCAM1 (R&D Systems; AF643-SP), rabbit Anti-VCAM1 (Abcam; ab134047), rabbit Anti-SPP1(Abcam, ab218237)]), mouse Anti-CFTR (Cystic Fibrosis Foundation, 596), rabbit Anti-GPRC5A (Novus Biologicals; NBP1-89743) and incubated overnight at 4°C. .. When CFTR was stained, The next day, slides were wash with PBS, and secondary antibodies in blocking buffer were added (1:200) [donkey Anti-Goat (Invitrogen; A11057 or A32814), Donkey Anti-Mouse (Invitrogen; A10037), donkey Anti-Rabbit (Invitrogen; A10042 or Jackson ImmunoResearch; 711-545-152), Anti-Sheep antibody (Jackson ImmunoResearch; 713-165-147), conjugated Streptavidin (Invitrogen; S21374) and incubated at room temperature for 1 h. Sections were stained with DAPI (4 ',6' -diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies).

    Incubation:

    Article Title: Supporting Information for Multiomics profiling of mouse polycystic kidney disease progression at a single-cell resolution
    Article Snippet: The slides were placed in antigen retrieval solution (Vector H-330) and incubated in a pressure cooker. .. The sections were blocked in blocking buffer [1% BSA (Roche; 03116956001), 0.1% Triton X-100 (Sigma; T8787) in PBS] for 1 h, and the following primary antibodies or glycoproteins were added (1:200); LTL (Vector Labs; B-1325 or FL1321), DBA (Vector Labs; B-1035 or FL-1031), sheep Anti-UMOD (Bio-Rad; 8595-0054), rabbit Anti-Calbindin1 (Abcam; ab108404), goat Anti-AQP2 (Novus Biologicals; NBP1-70378), rabbit Anti-TMSB4X (Immunodiagnostik; A9520.2), goat Anti-VCAM1 (R&D Systems; AF643-SP), rabbit Anti-VCAM1 (Abcam; ab134047), rabbit Anti-SPP1(Abcam; ab218237), rabbit Anti-GPRC5A (Novus Biologicals; NBP1-89743) and incubated overnight at 4°C. .. The next day, slides were wash with PBS, and secondary antibodies in blocking buffer were added (1:200); donkey Anti-Goat (Invitrogen; A11057 or A32814), donkey Anti-Mouse (Invitrogen; A10037), donkey Anti-Rabbit (Invitrogen; A10042 or Jackson ImmunoResearch; 711-545-152), donkey Anti-Sheep antibody (Jackson ImmunoResearch; 713-165-147), conjugated streptavidin (Invitrogen; S21374) and incubated at room temperature for 1 h. Sections were stained with DAPI (4 ,́6 ́- diamidino-2phenylindole) and mounted in Prolong Gold (Life Technologies).

    Article Title: Multi-omics profiling of mouse polycystic kidney disease progression at a single cell resolution
    Article Snippet: The slides were placed in antigen retrieval solution (Vector H-330) and incubated in a pressure cooker. .. The sections were blocked in blocking buffer [1% BSA (Roche; 03116956001), 0.1% Triton X-100 (Sigma; T8787) in PBS] for 1 h, and the following primary antibodies or glycoproteins were added (1:200); LTL (Vector Labs; B-1325 or FL-1321), DBA (Vector Labs; B-1035 or FL-1031), sheep Anti-UMOD (Bio-Rad; 8595-0054), rabbit Anti-Calbindin1 (Abcam; ab108404), goat Anti-AQP2 (Novus Biologicals; NBP1-70378), rabbit Anti-TMSB4X (Immunodiagnostik, A9520.2), goat Anti-VCAM1 (R&D Systems; AF643-SP), rabbit Anti-VCAM1 (Abcam; ab134047), rabbit Anti-SPP1(Abcam, ab218237)]), mouse Anti-CFTR (Cystic Fibrosis Foundation, 596), rabbit Anti-GPRC5A (Novus Biologicals; NBP1-89743) and incubated overnight at 4°C. .. When CFTR was stained, The next day, slides were wash with PBS, and secondary antibodies in blocking buffer were added (1:200) [donkey Anti-Goat (Invitrogen; A11057 or A32814), Donkey Anti-Mouse (Invitrogen; A10037), donkey Anti-Rabbit (Invitrogen; A10042 or Jackson ImmunoResearch; 711-545-152), Anti-Sheep antibody (Jackson ImmunoResearch; 713-165-147), conjugated Streptavidin (Invitrogen; S21374) and incubated at room temperature for 1 h. Sections were stained with DAPI (4 ',6' -diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies).

    Staining:

    Article Title: Multimodal spatial transcriptomic characterization of mouse kidney injury and repair
    Article Snippet: Kidneys were cryosectioned at 5-7μm thickness and mounted on Superfrost slides (Thermo Fisher Scientific). .. Sections were washed with PBS (three times, 5 min each), blocked and permeabilized with 1% BSA and 0.3% Triton X-100 in PBS for 1 hour then stained with primary antibody overnight in 4 o C. Primary antibodies (1:200) used were specific for rat anti-F4/80 (ab6640, Abcam), goat anti-Collagen 4a1/2 (1340-01, SouthernBiotech), rat anti-ICAM1 (BE0020-1, BioXCell), and rabbit anti-VCAM1 (EPR5047, Abcam), goat anti-VCAM1 (AF643-SP, R&D systems), rabbit anti-RUNX2 (12556S, Cell Signaling), FITC-conjugated ITGb2 (sc-8420, Santa Cruz), FITC-conjugated α-SMA (F3777, MilliporeSigma). .. Secondary antibodies (1:200) included AF488-, AF555-, or AF647 (Invitrogen).



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